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Three AFB’s should be ordered
Collected 8-hrs apart with ideally one specimen taken in am on waking)
If only 1 AFB ordered clarify with care team directly and ask to order 2 more
Consider collection of induced sputum for patients that are unable to produce good quality specimens or for whom bronchoscopy is not appropriate
Specimens should be of good quality; ask to recollect if lab reports specimen of poor quality
When interpreting AFB result, consider possible influence of previous/concurrent antibiotic use (e.g. Moxifloxicin)
Ask to collect post-bronchoscopy specimen. Label as “post-bronchoscopy specimen " or lab not to reject if < 8hrs since previous specimen