Blood Culture Volume

2 mL (may decrease volume depending on health status)
One site
Note: one aerobic (pink) and one anerobic (purple) top
Pediatric blood culture bottles with 1mL each

1-2 mL (may decrease volume depending on health status)
One site
2 (pink) Pediatric blood culture bottles with 0.5 to 1mL in each

2 mL
One site
1 (pink) Pediatric bottle with 2 mL

2 mL
One site (if no CVL)
If CVL (except PICC in NICU) obtain both CVL and peripheral culture if possible.
1 or 2 (pink) Pediatric bottles with 1 mL each or one with 2 mL

6 mL (may be decreased if at lower end of weight range)
One site if no CVL or if ≤ 5 days of life
If CVL and > 5 days of life, (except PICC in NICU) obtain both CVL and peripheral culture if possible
2 (pink) Pediatric blood culture bottles with at 1-3 mL in each or if <5mL, then 1 bottle

20 mls
Two sites
If CVL, obtain cultures from both CVL and peripheral sites
2 (blue) Adult Aerobic blood culture bottles with10 mL each

40 mls
Two sites
If CVL, obtain cultures from both CVL and peripheral sites
4 (blue) Adult Aerobic blood culture bottles with10 mL each